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Ageassociated declines in spermatogenesis are differentially therapy responsive

Western blot analysis Western blot analysis was performed according to the standard procedure to analyze the expression of FTH1 (1:1000, BOSTER, California, USA, #BM4487), NCOA4 (1:1000, Abcam, Cambridge, MA, USA, #ab86707), ATG5 (1:1000, BOSTER, #BA3525-2), -actin (1:2000, Abcam, #ab8226), TP53 (1:1000, BOSTER, #BM4309), CD63 (1:1000, Abcam, #ab193349), CD9 (1:1000, Abcam, #ab92726), and GAPDH (1:1000, Shanghai Siding Biotechnology Co., Ltd., China, #SD0033)

Cells were washed once with PBS, then centrifuge to collect the cells and discard the supernatant

This data reveals patterns that subjective memory would miss