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As a result, the increased presence of M1 macrophages not only contributes to immunotherapy, but also sustains a proinflammatory tumor microenvironment that supports tumor growth at early stage [137]

After harvesting, cells were plated to a density of 6 0.5 10 5 cells/mL in six-well plates (Corning) and allowed to incubate for 72 h at 37C in a 5% (v/v) CO 2 atmosphere to a confluence of 90%

Quantitative analysis was performed using ImageJ software by counting the number of Nissl-positive neurons in the hippocampal CA1, CA3, and dentate gyrus (DG) regions

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