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The mixture was shaken for 5 min for thorough mixing and centrifuged at 14,000 rpm for 10 min at 4 C, and the supernatants were directly transferred into specific glass vials until assayed

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Moreover, patient-specific lung organoids, derived from induced pluripotent stem cells (iPSCs) or adult tissue stem cells, cultured into microfluidic devices, also allow for the creation of physiologically relevant systems that better represent individual patient characteristics (71, 160)

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