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IL-6 and CCL-2 in WAT of WT mice but not db/db mice [1]
the concentration of extracted protein is usually low
The addition of ubiquitin to a substrate protein is called ubiquitination or ubiquitylation
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Rat INS (Insulin) ELISA Kit anti-Nampt IL-6 and CCL-2 in WATThis kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti INS antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with INS bound to precoated antibody. Wash unbound components and add HRP Streptavidin Conjugate (SABC). Wash
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