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Some experimental structures explore: 48 week research durations Periodic non-research intervals between protocols Storage & Handling Store refrigerated at 28C Protect from excessive heat and sunlight Use clean laboratory handling practices Avoid repeated contamination of the vial Important Disclaimer For Laboratory and Research Purposes Only Not intended for human consumption or therapeutic use

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coli cells were grown in LB medium at 37 C until an OD of 0.6 was reached and then induced by adding 0.2 mM IPTG at 18 C overnight