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Bradford Protein Assay for the Quantification of Total Protein LDH, caspase 3, SOD and GPx activities, the levels of DCFDA, 8-OH-dG, TBARS, DNPH, GSSG/GSH and several cytokines, and TAC were adjusted for variations in cell number using the Bradford protein assay described by Bradford ( Data Analysis The results obtained are presented as the means the standard error of measurement (SEM)

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In SIM mode, the fragment ion with an m/z of [M-57] (where M represents the molecular mass of each derivatized metabolite, and 57 corresponds to the loss of the tert-butyl moiety during GC analysis) was designated as the quantitative ion for each compound