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Real-time quantitative PCR Total RNA was extracted from aortic tissue and endothelial cells using TRIzol reagent (Takara Bio Inc., 9108), following the manufacturers instructions

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The search strategy employed combinations of keywords and MeSH terms including: diabetes mellitus , male infertility , spermatogenesis , oxidative stress , epigenetics , endocrine dysfunction , and sperm quality

Biochem J 468:7385 Lescot M, Dhais P, Thijs G et al (2002) PlantCARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences