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The model was improved through iterative cycles of manual model building with Coot and refinement with REFMAC in the CCP4 suite 48,49

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For instance, 3D structure-based nano-in-microparticles synthesized using polysaccharides such as maltodextrin or dextran, amino acids, and doped with copper oxide (CuO) or zinc oxide (ZnO) NPs are used for skin repair and to prevent bacterial infection by overcoming colonization

After 28 days the benzyl alcohol preservative is no longer considered reliable, so the safest choice is to discard the vial and open a new one, even if the water still looks clear