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Among the 14 identified candidates, acetylcarnitine was finally selected by confirming whether it was endogenous in the blood, matched with the mass value, and exhibited a significant difference in intensity between the MDD and control groups (Figure 4)

As expected, the induction of primitive streak and intermediate mesoderm was initiated by treatment with CHIR99021 (a Wnt agonist) and Noggin (a BMP inhibitor), the former essential for mesoderm lineage specification and the latter represses lateral plate mesoderm differentiation [11]

In cultured human fibroblasts and other cell models, exposure to GHK-Cu has been associated with changes in gene expression profiles related to cellular stress responses, growth signaling, and matrix-associated proteins[4]

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