Numerous factors, such as a poor diet, chronic illness, infection, and ongoing stress, can cause your body's glutathione level to drop
When mice reached approximately 20 weeks of age, they were single housed and food intake was daily measured for 5 to 7 consecutive days
Subsequently, fertilized oocytes from each group were transferred individually to embryo culture droplets as SOF medium supplemented with 0.5 mM sodium citrate, 1 mM sodium pyruvate, 1% non-essential amino acids, 2% essential amino acids, 2 mM Gluta MAX , 2.8 mM myo-inositol and 4 mg/ml bovine serum albumin covered by mineral oil in a 35 mm sterile dish, followed by embryo culture in a CO 2 incubator (38.5C, 5%O 2 and 5%CO 2 ) ( After 48 h, cleaved two-cell embryos from each group were microscopically observed with cleavage rates calculated as the percentage of cleaved two-cell embryos/MII oocytes
Kennedy RS, Konok GP, Bounous G, Baruchel S, Lee TD