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To identify the specific effects of ferroptosis on ESCs, primary ESCs were treated with several ferroptosis inducers, such as erastin (30 M), (1S,3R)-RSL3 (10 M), tert-butyl hydroperoxide solution (TBHP, 20 M), diluted cyst fluid (1:1 dilution with complete medium), and with inhibitor NAC (10 M) for 12 h

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